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991.
C Gerardin-Charbonnier S Auberger L Molina S Achilefu M A Manresa P Vinardell M R Infante C Selve 《Preparative biochemistry & biotechnology》1999,29(3):257-272
A series of diverse beta-lactam analogues of nocardicins with interesting antimicrobial properties were prepared. Coupling of glucosamine to these compounds improved their water solubility. Aminoacid derivatives produced a stereoinduction on the quaternary enantiotopic carbon of the starting compound 1. Evaluation of their antimicrobial activity showed that the introduction of alpha-amninoacids to monobactams increased their activity. The importance of asymmetric carbon is exemplified by the higher antibiotic activity of L-alpha-aminoacids than the D-series. No significant difference was observed between fluorinated and non-fluorinated monobactams. 相似文献
992.
Starburst glycodendrimers offer the potential to serve as high-affinity ligands for clinically relevant sugar receptors. In order to define areas of application, their binding behavior towards sugar receptors with differential binding-site orientation but identical monosaccharide specificity must be evaluated. Using poly(amidoamine) starburst dendrimers of five generations, which contain the p-isothiocyanato derivative of p-aminophenyl-beta-D-lactoside as ligand group, four different types of galactoside-binding proteins were chosen for this purpose, i.e., the (AB)(2)-toxic agglutinin from mistletoe, a human immunoglobulin G fraction, the homodimeric galectin-1 with its two binding sites at opposite ends of the jelly-roll-motif-harboring protein and monomeric galectin-3. Direct solid-phase assays with surface-immobilized glycodendrimers resulted in obvious affinity enhancements by progressive core branching for the plant agglutinin and less pronounced for the antibody and galectin-1. High density of binding of galectin-3 with modest affinity increases only from the level of the 32-mer onwards points to favorable protein-protein interactions of the monomeric lectin and a spherical display of the end groups without a major share of backfolding. When the inhibitory potency of these probes was evaluated as competitor of receptor binding to an immobilized neoglycoprotein or to asialofetuin, a marked selectivity was detected. The 32- and 64-mers were second to none as inhibitors for the plant agglutinin against both ligand-exposing matrices and for galectin-1 on the matrix with a heterogeneous array of interglycoside distances even on the per-sugar basis. In contrast, a neoglycoprotein with the same end group was superior in the case of the antibody and, less pronounced, monomeric galectin-3. Intimate details of topological binding-site presentation and the ligand display on different generations of core assembly are major operative factors which determine the potential of dendrimers for applications as lectin-targeting device, as attested by these observations. 相似文献
993.
A. G. Jeffs M. E. Willmott R. M. G. Wells 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》1999,123(4):107
Nektonic pueruli of the spiny lobster, Jasus edwardsii, were caught from two locations about 20 km apart across the continental shelf on the south east of the North Island, New Zealand. The pueruli were assayed for total protein, glucose, glycogen, and lipid content. Only the lipid content differed between pueruli caught onshore and offshore (mean difference=3.1 mg or 3.4% of dry mass). The average difference in lipid content measured over this distance was used to calculate the rate of energy consumption and timing for pueruli to actively swim from the continental shelf to shore. These results confirmed previous theoretical estimates and indirect measures. Furthermore, the rate of energy consumption would allow all of the pueruli caught offshore to swim to shore based on their total measured lipids. However, some individuals with low energy stores may be energetically compromised at arrival which may affect their subsequent moulting and survival. The results of this study indicate that lipid is the primary format for energy storage of the nektonic puerulus of the spiny lobster and that these lipid reserves have sufficient energetic capacity to allow the puerulus to actively swim the distance across the shelf to settle on the coast. 相似文献
994.
R. Zárate 《Plant cell reports》1999,18(5):418-423
Atropa baetica hairy root cultures were induced after infecting stem segments with Agrobacterium rhizogenes strain ATCC 15834. Accumulation of the tropane alkaloids atropine and scopolamine by hairy roots cultured in half- and full-strength
Murashige and Skoog (MS) medium was high, although this was not growth associated. These alkaloids were also released into
both liquid media. Higher tropane alkaloids present both in hairy roots and liquid medium occurred in half MS medium, showing
a clear relationship between slow growth of cultures and higher product accumulation. The pH of both nutrient media varied
as culture progressed, and seemed to be associated with the release of scopolamine. GC-MS analyses showed the presence of
a new compound, namely tigloylpseudotropine; moreover, 3α-isobutyryloxytropane, formerly found only in plant leaf tissue,
was also identified in the hairy roots.
Received: 18 August 1997 / Revision received: 30 November 1997 / Accepted: 20 January 1998 相似文献
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999.
Morphological variation in intergrade pupfish populations from the Pecos River, Texas, U.S.A. 总被引:1,自引:0,他引:1
In the early 1980s, sheepshead minnow Cyprinodon variegates was introduced into the Pecos River, Texas, U.S.A. where it hybridized with the endemic Pecos pupfish C. pecosensis . By 1985, pupfish populations throughout approximately 300 km of the river consisted exclusively of individuals of hybrid origin (intergrades). There was significant ( P <0·05) geographic variation in most morphological characters; the general pattern of variation was of a bidirectional cline centred near Pecos, Texas. At that site, morphology of intergrade populations resembled mostly that of the introduced species. Upstream and downstream from Pecos, morphology shifted progressively toward that typical of the native form. Intergrade populations were morphologically intermediate to the parental forms, showed a rapid approach to random assortment of characters, and generally exhibited greater morphological variability than occurred in either parent species. These observations and the consistent lack of bimodality in frequency distributions of a morphological hybrid index support the contention that intergrade populations comprise panmictic admixtures of C. variegates and C. pecosensis . 相似文献
1000.
Olfactory versus visual cues in a floral mimicry system 总被引:5,自引:0,他引:5
We used arrays of artificial flowers with and without fragrance to determine the importance of olfactory and visual cues
in attracting insects to a floral mimic. The mimic is a fungus, Puccinia
monoica Arth., which causes its crucifer hosts (here, Arabis
drummondii Gray) to form pseudoflowers that mimic co-occurring flowers such as the buttercup, Ranunculus
inamoenus Greene. Although pseudoflowers are visually similar to buttercups, their sweet fragrance is distinct. To determine whether
visitors to pseudoflowers were responding to fragrance we performed an experiment in which we removed the visual cues, but
allowed fragrance to still be perceived. In this experiment we found that pseudoflower fragrance can attract visitors by itself.
In other experiments we found that the relative importance of olfactory and visual cues depended on the species of visitor.
Halictid bees (Dialictus sp.) had a somewhat greater visual than olfactory response, whereas flies (muscids and anthomyiids) were more dependent on
olfactory cues. We also used bioassays to determine which of the many compounds present in the natural fragrance were responsible
for attraction. We found that halictid bees were equally attracted to pseudoflowers and to a blend containing phenylacetaldehyde,
2-phenylethanol, benzaldehyde and methylbenzoate in the same relative concentrations as in pseudoflowers. Flies, on the other
hand, only responded to pseudoflower scent, indicating that we have not yet identified the compound(s) present in pseudoflowers
that are attracting them. The ability of insects to differentiate pseudoflowers from true flowers by their fragrance may be
important in the evolution of the mimicry system. Different fragrances may facilitate proper transfer of both fungal spermatia
and pollen, and thus make it possible for the visual mimicry to evolve.
Received: 3 January 1996 / Accepted: 13 August 1996 相似文献